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mouse egr1 fam  (Thermo Fisher)


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    Thermo Fisher mouse egr1 fam
    EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of <t>Egr1</t> from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.
    Mouse Egr1 Fam, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse egr1 fam/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mouse egr1 fam - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells"

    Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

    Journal: iScience

    doi: 10.1016/j.isci.2024.110306

    EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of Egr1 from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.
    Figure Legend Snippet: EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of Egr1 from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.

    Techniques Used: Saline, Isolation, Sequencing, Expressing, RNA Sequencing, Cell Culture


    Figure Legend Snippet:

    Techniques Used: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, Software



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    mouse egr1 fam  (Thermo Fisher)
    90
    Thermo Fisher mouse egr1 fam
    EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of <t>Egr1</t> from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.
    Mouse Egr1 Fam, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse egr1 fam/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    mouse egr1 fam - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of Egr1 from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.

    Journal: iScience

    Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

    doi: 10.1016/j.isci.2024.110306

    Figure Lengend Snippet: EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of Egr1 from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.

    Article Snippet: Mouse Egr1 FAM , ThermoFisher , Mm00656724_m1.

    Techniques: Saline, Isolation, Sequencing, Expressing, RNA Sequencing, Cell Culture

    Journal: iScience

    Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

    doi: 10.1016/j.isci.2024.110306

    Figure Lengend Snippet:

    Article Snippet: Mouse Egr1 FAM , ThermoFisher , Mm00656724_m1.

    Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, Software